Classic Style Guide,High-affinity competitive elution of FLAG-tagged proteins

In the realm of molecular biology and protein research, precise tools are paramount for accurate experimentation and reliable results. The 3x FLAG peptide has emerged as a highly valuable asset, particularly the offerings from APEXBio. This article delves into the specifics of the 3x FLAG peptide from APEXBio, exploring its composition, applications, and the verifiable information surrounding its use in scientific endeavors.

Understanding the 3x FLAG Peptide

The 3X FLAG peptide is a synthetic polypeptide characterized by the presence of three tandem repeats of the sequence DYKDDDDK. This specific amino acid sequence, often referred to as the DYKDDDDK peptide or 3X (DYKDDDDK) Peptide, functions as an epitope tag. Epitope tags are short amino acid sequences that are fused to a protein of interest, allowing for its detection, purification, and manipulation using specific antibodies. The 3X FLAG peptide's design, with its triple repetition, enhances the binding affinity to anti-FLAG antibodies, making it a robust tool for various biological applications.

Key Characteristics and Specifications

APEXBio provides the 3X FLAG peptide with detailed specifications that are crucial for researchers to consider. For instance, the Product Name: 3X FLAG Peptide is cataloged under A6001. The 3X FLAG peptide from APEXBio is reported to have a purity of 98.66%. It is typically supplied in a concentration of ≥25mg/ml in TBS (0.5M Tris-HCl, pH 7.4, with 1M NaCl). The molecular weight of this peptide is approximately 2861.87 Da. Researchers can acquire this essential reagent in various quantities, such as 3x Flag Peptide 4 mg or 3x Flag Peptide 25 mg, with pricing varying accordingly. For example, the 3x Flag Peptide 4 mg is listed at approximately $108, while the 3x Flag Peptide 25 mg is priced around $780, demonstrating the cost-effectiveness often associated with APEXBio's offerings, as highlighted in discussions comparing it to other suppliers.

Applications in Biological Research

The 3X FLAG peptide is widely utilized in biological research due to its versatility. Its primary applications include:

* Protein Purification: The 3X FLAG peptide is instrumental in affinity purification techniques. By fusing this tag to a recombinant protein, researchers can selectively capture and isolate the protein of interest from complex biological mixtures using anti-FLAG antibodies immobilized on a solid support. This method ensures high-purity protein for downstream analyses.

* Immunodetection: The tagged protein can be readily detected in various assays, such as Western Blotting (WB) and Mass Spectrometry (Mass Spec), by employing antibodies that specifically recognize the FLAG epitope.

* Protein Localization and Interaction Studies: The ability to specifically detect and isolate FLAG-tagged proteins aids in studying their cellular localization and interactions with other biomolecules.

* Competitive Elution: The 3X FLAG peptide can be used for high-affinity competitive elution of FLAG-tagged proteins. This method allows for the gentle release of the purified protein from the antibody-bound resin, preserving its native conformation, which is often preferable to harsher elution methods.

Expert Insights and Verifiable Information

APEXBio's commitment to quality is underscored by its manufacturing processes, which adhere to stringent quality controls, ensuring batch-to-batch consistency. This reliability is vital for reproducible scientific outcomes. The 3X (DYKDDDDK) Peptide from APEXBio has been cited in numerous publications, attesting to its widespread adoption and proven performance in demanding research environments. The 3X FLAG peptide's unique properties empower researchers in applications ranging from rapid screening to high-resolution structural studies.

Conclusion

The 3x FLAG peptide from APEXBio, identified by its catalog number A6001, stands as a reliable and effective tool for a multitude of molecular biology applications. Its well-defined sequence, high purity, and the proven track record of APEXBio as a supplier make it an excellent choice for researchers seeking to advance their work in protein purification, detection, and structural analysis. Whether you are exploring 3x FLAG peptide apexbio price options or seeking detailed product information, APEXBio offers a robust solution for your research needs.